支原体（一）.ppt

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　　标题： Mycoplasma支原体

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　　标题：

　　正文： Defination Discovery Classification Morphology Structure Cultural character Compassion with L-formed Bacterium

　　Content

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　　标题： Mycoplasma

　　正文： No cell wall Prokaryotic microbe free-living in nature Can live in the non-living medium Bacteria-filtration device Have filament with branches

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　　标题： Model

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　　标题： Discovery

　　正文： In 1898, Nocard found Mycoplasma from the cattle that had pleuropneumonia . In 1937, Dienes isolated Mycoplasma from the pus of patients. Once they were named pleuropneumonia-like organism (类胸膜肺炎微生物) In 1962, Chanock succeeded in cultivating Mycoplasma aitificially.

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　　标题： Classification

　　正文： Mycoplasma belongs to mollicute(柔膜体纲)Mycoplasmatales(支原体目). Mycoplasmataceae(支原体科) Ancholeplasmataceae(无胆淄原体科) Spiroplasmataceae(螺原体科)

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　　标题： Mycoplasmataceae(支原体科)

　　正文： Mycoplasma(支原体属) * M.pneumoniae(肺炎支原体) * M.hominis(人型支原体) * M.genitalium(生殖器支原体) Ureaplasma(脲原体属) * U.urealyticum(溶脲脲原体)

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　　M.pneumoniae

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　　标题： 人类主要支原体生物学性状

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　　标题： Shape of mycoplasma

　　正文： Size: The smallest, 0.2 ~ 0.3 μ m, under pressure it can pass though Bacteria-filtration device. Usually spherical, double spherical, filamentous Vegetative mode: binary fission Stain: hard to be stained in Gram. Giemsa, blue

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　　M.Pneumoniae with Giemsa stain

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　　标题： Structure (Under electron microscope )

　　正文： Three layers: outer and inner layers contain protein and glycogen, meddle layer is composed of lipid (cholesterol takes up 36%) The only cellular organ –ribosome. DNA and RNA, gene is dsDNA.

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　　正文： Special structures: Some contain terminal structure(顶端结构), related to the adhesion. Capsule, has something to do with virulence. Microfilament

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　　标题： Cultivation

　　正文： high Nutritional requirements : cardin (牛心浸液),serum, yeast steep(酵母浸液) In pH7.8 ~ 8.0 growth below 7.0 deaths (Ureaplasma urealyticum pH6.0 ~ 6.5) Need 5%~10%CO2 Slow Growth，life cycle: 1~3h liquid medium: Difficult to see the turbid Solid medium: A typical colony was fried egg shape. U.urealyticum:10~40 μ m, like "T" line

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　　标题： Antigen structure

　　正文： Antigen structure: membrane protein ( use ELISA to detect) and glycolipids. Glycolipids have strong antigens, but have poor specificity. Part of M.pneumoniae has capsule, which is mainly polysaccharide. Some have mitogen, cause autoimmune reaction.

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　　标题： Resistance

　　正文： Death 50℃ 30minutes or 55℃ 5-15 minutes. Sensitive to UV, dry, heat, Cleanser and disinfectant. Strong resistance to: low temperature(-70 ℃), thallium acetate(铊酸), crystal violet(结晶紫). Resistance to penicillin, etc. The antibiotic-sensitive protein synthesis interference.

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　　标题： Both the mycoplasma and the L –formed bacterium don't have the cell wall, their biological characters are similar. But what’s the Differences between them?

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　　标题： Difference between L-formed and M.

　　其他占位符：

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　　Mycoplasma colony

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　　L-formed colony

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　　Thank you!!!~